Mikrodalga �n i�leminden ge�irilmi� at�k kuru incirin biyohidrojen �retimi i�in de�erlendirilmesi

�zm�h��, Serpil; Hac�o�lu, �lknur

Cilt : 30 Say� : 2 Y�l : 2024

30/2Son Say� Erken Bask� Ar�iv Pop�ler Makaleler Makale G�nderimi

Mikrodalga �n i�leminden ge�irilmi� at�k kuru incirin biyohidrojen �retimi i�in de�erlendirilmesi [Pamukkale Univ Muh Bilim Derg]

Pamukkale Univ Muh Bilim Derg. 2023; 29(7): 760-768 | DOI: 10.5505/pajes.2023.23238

Mikrodalga �n i�leminden ge�irilmi� at�k kuru incirin biyohidrojen �retimi i�in de�erlendirilmesi

Serpil �zm�h��¹, �lknur Hac�o�lu²
¹Dokuz Eyl�l �niversitesi, M�hendislik Fak�ltesi, �evre M�hendsili�i B�l�m�
²Dokuz Eyl�l �niversitesi, Fen Bilimleri Enstit�s�, Biyoteknoloji Anabilim Dal�

�al��mada; at�k kuru incirde mikrodalga �n i�lemi (250-800W) uygulanarak aflotoksin giderimi ve en y�ksek ��z�nm�� eker eldesi i�in en iyi ko�ullar� saptanm��t�r. Aflatoksini giderilmi� at�k kuru incirlerin biyohidrojen �retim performanslar� farkl� substrat (11.8-118 g/L) ve farkl� organizma konsantrasyonlar� (0.5-2.5 g/L) denenerek test edilmi�tir. Mikrodalga i�lemi sonucunda 100 g/L at�k kuru incirden en y�ksek ��z�nm�� eker konsantrasyonunu (89.7 g/L) veren dalga boyu 400 W ve muamele s�resi 10 dk. olarak bulunmu�tur. Bu ko�ullarda %46.2 �eker hidrolizlenmesi ve % 77.8 aflatoksin giderimi sa�lanm��t�r. En y�ksek k�m�latif hidrojen gaz� hacmi, hidrojen gaz� �retim verimi, hidrojen gaz� �retim h�z�; 59 g/L ba�lang�� substrat konsantrasyonunda s�ras�yla, 161.9 mL, 207.5 mL H2/g toplam �eker, 1.75 mL/saat olarak bulunmu�tur. Farkl� substrat konsantrasyonlar�n�n kullan�ld�� deneylerde �retilen organik asitlerden, laktik asidin y�ksek miktarlarda �retiliyor olmas�na ra�men, baz� deneylerde b�trik aside d�n��t�� g�zlemlenmi�tir. Bu da biyohidrojen gaz� �retimlerinde art�� sa�lam��t�r. Organizma miktar� de�i�im deneylerinde �ekerin t�m deney ortamlar�nda t�ketildi�i g�zlenmi� ve ba�ar�l� bir �ekilde hidrojen gaz� �retimi ger�ekle�mi�tir. Yo�unluklu olarak b�trik asit �retilen deney ortamlar�nda en y�ksek hidrojen gaz� �retim verimi 1 g/L organizma i�eren deney ortam�nda 124.28 mL H2/g toplam �eker olarak elde edilmi�tir. Organizma konsantrasyonu artt�k�a spesifik hidrojen �retim h�z�nda [SH�H) d�� g�zlenmi�tir. En y�ksek SH�H, 0.5 g/L organizma i�eren fermantasyon ortam�nda 3.83 mL H2/g biyok�tle saat olarak bulunmu�tur.

Anahtar Kelimeler: Aflatoksin, At�k Kuru �ncir, Biyohidrojen �retimi, Substrat Konsantrasyonu, Organizma Konsantrasyonu,

Evaluation of microwave pretreated waste dry fig for biohydrogen production

Serpil �zm�h��¹, �lknur Hac�o�lu²
¹University Of Dokuz Eyl�l, Engineering Faculty, Environmental Engineering Department
²University Of Dokuz Eyl�l, Graduation School Of Natural And Applied Sciences, Biotechnology Department

In the study, the best conditions for aflatoxin removal and the highest dissolved sugar were determined by applying microwave pretreatment (250-800W) to waste dried figs. Biohydrogen production performances of aflatoxin-free dried figs were evaluated by testing different substrate (11.8-118 g/L) and different organism concentrations (0.5-2.5 g/L). The optimum microwave of the microwave pretreatment process, giving the highest dissolved sugar concentration (89.7 g/L) from 100 g/L waste dried figs was 400 W and the treatment time was 10 minutes. Under these conditions, 46.2% hydrolysis of sugars and 77.8% aflatoxin removal were achieved. At the initial substrate concentration of 59 g/L, the highest cumulative volume of hydrogen gas, hydrogen gas production yield, hydrogen gas production rate were found as 161.9 mL, 207.5 mL H2/g total sugar, 1.75 mL/hour, respectively. Although lactic acid was produced in high amounts from organic acids produced in experiments using different substrate concentrations, it has been observed that it is converted into butyric acid in some experiments. This resulted as an increase in biohydrogen gas production. In different organism amount experiments, it was observed that sugar was consumed in all experiments and hydrogen gas was produced successfully. Butyric acid was the main organic acid in the experimental media where the highest hydrogen gas production yield was produced in 1 g/L as 124.28 mL H2/g total sugar. The specific hydrogen gas production rate (SHPR) decreased with increasing organism concentrations. The highest SHPR was obtained with 0.5 g/L organism concentration as 3.83 mL H2/g biomass hour.

Keywords: Aflatoxin, Waste Dry Fig, Biohydrogen Production, Substrate Concentration, Organism Concentration

Serpil �zm�h��, �lknur Hac�o�lu. Evaluation of microwave pretreated waste dry fig for biohydrogen production. Pamukkale Univ Muh Bilim Derg. 2023; 29(7): 760-768

Sorumlu Yazar: Serpil �zm�h��, T�rkiye
Makale Dili: T�rk�e

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